Forms Data Controls :: GridView Column Set Fixed Width

I am trying to set the width of each column within my gridview. Problem is that none of it is working. I have tried setting the whole grid view to 100% and then break down each individual column to a certain percentage, tried manually adjusting Header Width, Item Style width, tried setting it in code, I have tried just about everything and the column doesn't want to budge. The only time I can adjust the width of the column/cell is when I do it in SQL, but because they are requesting the tooltip to preview the comment I would like to pursue my options doing it on the page itself.

//Code protected void grdComments_RowDataBound(object sender, GridViewRowEventArgs e)
{//Setting the tooltip for the comment column and column width.
grdComments.Columns[3].ItemStyle.Width = Unit.Pixel(100);
//e.Row.Cells[3].Width = Unit.Pixel(100);
e.Row.Cells[3].Wrap = false;
e.Row.Cells[3].Style["overflow"] = "hidden";
e.Row.Cells[3].Style["text-overflow"] = "ellipsis";
e.Row.Cells[3].ToolTip = e.Row.Cells[3].Text; }